Comparison of heavy metals, secondary metabolites, and total polyphenols in hypericum perforatum l. and althaea officinalis L

Memushaj, Lorena; Shtëmbari, Afërdita; Keri, Jona

doi:10.48309/jmpcr.2024.449445.1148

Articles in Press

Document Type : Original Research Article

Authors

¹ Department of Pharmacy, Faculty of Medical Sciences, Aldent University, Tiranë, Albania

² Department of Industrial Chemistry, Faculty of Natural Sciences, University of Tirana, Tirana, Albania

10.48309/jmpcr.2024.449445.1148

Abstract

Hypericum perforatum L. and Althaea officinalis L. are medicinal plants of interest to the pharmaceutical, health, and food industry, as well as the study of their chemical composition is very important to evaluate the most influential components against their importance in the treatment of diseases. The purpose of this study was the comparison of the secondary metabolites, heavy metals, and total polyphenols. The heavy metals are determined by inductively coupled plasma mass spectrometry. The obtained extracts were analyzed using Gas Chromatography Flame Ionization Detector. Based on the result, it can be seen that the element that has the most difference in value is lead and cadmium, respectively 0.2025 mg/kg (Pb) and 0.375 mg/kg (Cd) for H. perforatum L.;0.41 mg/kg (Pb) and 0.142 mg/kg for A.officinalis L. The compounds that have the most differences compared to Hypericum perforatum L. and Althaea officinalis L. extracts are: Tricyclene (26.706%; 0.142%); alpha-Pinene (15.613%; 0.044%); beta-Pinene (2.262%; 0.132%); cis-beta-Terpineol (0.826%; 0.334%); Caryophyllene-E (9.398%; 11.122%); alpha-Humulene (5.920%; 10.480%); and Ledol (0.674%; 1.356%). The total polyphenol (TP) content of Hypericum perforatum L. is 7.25% (w/w) Gallic acid and 0.56% (w/w) Gallic acid for Althaea officinalis L.. As we mentioned, H. perforatum L. has a higher level of total polyphenols. Our results demonstrated that the two medicinal plants had a variety of chemical compounds with promising qualities that could lead to in-depth investigation into their possible uses in cosmetic and pharmaceutical fields. Further trials pertaining to the application of these two medicinal plants' essential oils and extracts may originate from this research.

Graphical Abstract

Keywords

Main Subjects

Herbal medicine

References

[1] J. Asgarpanah, Phytochemistry, pharmacology and medicinal properties of Hypericum perforatum L, African Journal of Pharmacy and Pharmacology, 2012, 6, 1387-1394. [Crossref], [Google Scholar], [Publisher]‎

[2] A. Alahmad, I. Alghoraibi, R. Zein, S. Kraft, G. Dräger, J.G. Walter, T. Scheper, Identification of major constituents of Hypericum perforatum L. extracts in Syria by development of a rapid, simple, and reproducible HPLC-ESI-Q-TOF MS analysis and their antioxidant activities, ACS Omega, 2022, 7, 13475-13493. [Crossref], [Google Scholar], [Publisher]‎

[3] V. Hadzhiiliev, D. Dimov, Separate isolation of hyperforin from hypericum perforatum (St. John’s Wort) pursuant to the coefficents LOG Kow, PKa and densities of the included compounds, Trakia Journal of Sciences, 2015, 13, 19-23. [Crossref], [Google Scholar], [Publisher]‎

[4] J. Patocka, The chemistry, pharmacology, and toxicology of the biologically active constituents of the herb Hypericum perforatum L, Journal of Applied Biomedicine, 2003, 1, 61-70. [Crossref], [Google Scholar], [Publisher]‎

[5] L. Verotta, G. Appendino, J. Jakupovic, E. Bombardelli, Hyperforin analogues from St. John's wort (Hypericum perforatum), Journal of Natural Products, 2000, 63, 412-415. [Crossref], [Google Scholar], [Publisher]‎

[6] M. Mahboubi, Marsh mallow (Althaea officinalis L.) and its potency in the treatment of cough, Complementary Medicine Research, 2020, 27, 174-183. [Crossref], [Google Scholar], [Publisher]‎

[7] S.S. Gautam, S. Kumar, R. Chauhan, Antimicrobial efficacy of Althaea officinalis Linn. seed extracts and essential oil against respiratory tract pathogens, Journal of Applied Pharmaceutical Science, 2015, 5, 115-119. [Crossref], [Google Scholar], [Publisher]‎

[8] M. Valiei, A. Shafaghat, F. Salimi, Chemical composition and antimicrobial activity of the flower and root hexane extracts of Althaea officinalis in Northwest Iran, Journal of Medicinal Plants Research, 2011, 5, 6972-6976. [Crossref], [Google Scholar], [Publisher]‎

[9] N. Adiputra, I.G.M. Aman, I.B.P. Manuaba, The toxicity of antiviral plants used in Balinese traditional medicine, Bali Medical Journal, 2017, 6, 243-246. [Crossref], [Google Scholar], [Publisher]‎

[10] S. Kilic, I.B. Cam, T. Tongur, M. Kilic, Health risk assessment of exposure to heavy metals and aflatoxins via dietary intake of dried red pepper from marketplaces in Antalya, Southern Turkey, Journal of Food Science, 2018, 83, 2675-2681. [Crossref], [Google Scholar], [Publisher]‎

[11] H. Mawarti, M. Rajin, Z. Khusniyah, Z. Asumta, C.D.W. Wijayanti, Aloe vera and its potency as antituberculosis against strains of Mycobacterium tuberculosis that is resistant to some tuberculosis drugs, Bali Medical Journal, 2022, 11, 1879-1883. [Crossref], [Google Scholar], [Publisher]‎

[12] M. Babu-Kasimala, M. Tukue, R. Ermias, Phytochemical screening and antibacterial activity of two common terresterial medicinal plants Ruta chalepensis and Rumex nervosus, Bali Medical Journal, 2014, 3, 116-121. [Crossref], [Google Scholar], [Publisher]‎

[13] P.H. Laswati, I., Subadi, M. Andriana, A,P. Maria, J. Alex Pangkahila, Tomato (Lycopersicum Commune) juice and physical exercise increase number of neurons and ERβ expression in post-ovariectomy rats brain, Bali Medical Journal (Bali Med J), 2016, 5, 84-91. [Crossref], [Google Scholar], [Publisher]‎

[14] A. Cakir, M.E. Duru, M. Harmandar, R. Ciriminna, S. Passannanti, F. Piozzi, Comparison of the volatile oils of Hypericum scabrum L. and Hypericum perforatum L. from Turkey, Flavour and Fragrance Journal, 1997, 12, 285-287. [Crossref], [Google Scholar], [Publisher]‎

[15] A. Shabani, M. Karapandzova, I.C. Karanfilova, G. Stefkov, S. Kulevanova, GC-MS analysis of the essential oil, aroma components and n-hexane extract of St. John Wort (Hypericum perforatum L., Hypericaceae), Macedonian Pharmaceutical Bulletin, 2018, 64. [Crossref], [Google Scholar], [Publisher]‎

[16] Q. Du, Y. Cai, Z. Chen, D. Wei, Y. Cao, Y. Chen, S. Yu, Q. Zhao, J. Wu, M. Liu, Determination of Trace Elements in Corydalis conspersa and Corydalis linarioides by ICP-AES, Journal of Chemistry, 2020, 2020, 1-11. [Crossref], [Google Scholar], [Publisher]‎

[17] Y.Q. Liu, X. Shao, L.L. Qu, ICP-AES determination of 11 kinds of elements in high grade pipeline steel, Advanced Materials Research, 2012, 455, 830-833. [Crossref], [Google Scholar], [Publisher]‎

[18] L.H. Jia, Y. Liu, Y.Z. Li, Determination of the major metal elements including heavy metals in Saffron from Tibet and Henan by ICPAES or ICP-MS, Journal of Chinese Pharmaceutical Sciences, 2011, 20, pp.297-301. [Crossref], [Google Scholar], [Publisher]‎

[19] M.I. Prawira-Atmaja, H.S. Khomaini, H. Maulana, S. Harianto, D. Rohdiana, March. Changes in chlorophyll and polyphenols content in Camellia sinensis var. sinensis at different stage of leaf maturity, In IOP Conference Series, Earth and Environmental Science IOP Publishing, 2018, 131, 12010. [Crossref], [Google Scholar], [Publisher]‎

[20] R. Aditya, B. Santoso, W. Widjiati, Comparative study of bioactive compound content and antioxidant activity in different extraction methods of Syzygium Polyanthum leaves, Bali Medical Journal, 2023, 12, 3425-3430.[Crossref], [Google Scholar], [Publisher]‎

[21] N.A. Korotkova, V.B. Baranovskaya, K.V. Petrova, Microwave digestion and ICP-MS determination of major and trace elements in waste Sm-Co magnets, Metals, 2022, 12, 1308. [Crossref], [Google Scholar], [Publisher]‎

Journal of Medicinal and Pharmaceutical Chemistry Research

Comparison of heavy metals, secondary metabolites, and total polyphenols in hypericum perforatum l. and althaea officinalis L

References

References

Articles in Press, Accepted Manuscript
Available Online from 05 May 2024

Comparison of heavy metals, secondary metabolites, and total polyphenols in hypericum perforatum l. and althaea officinalis L

References

References

Articles in Press, Accepted Manuscript Available Online from 05 May 2024

Articles in Press, Accepted Manuscript
Available Online from 05 May 2024