Authors

1 Department of Pharmaceutical Biology, Universitas Sumatera Utara, Medan, 20222, Indonesia

2 Department of Pharmaceutical Technology, Universitas Sumatera Utara, Medan, 20222, Indonesia

3 Department of Pharmacology, Universitas Sumatera Utara, Medan, 20222, Indonesia

4 Department of Pharmaceutical Chemistry, Universitas Sumatera Utara, Medan, 20222, Indonesia

Abstract

One of the plants that potential to develop as an anticancer agent is Duku leaf (Lansium domesticum Corr.). From previous studies, Duku leaf extract had cytotoxic activity against several cancerous cell lines. The aim of this study was to determine the cytotoxic activity of crude and purified extracts of Duku leaf against MCF-7 and HTB-183 cell lines. The crude extract of Duku leaf was obtained using maceration in ethanol absolute. The vacuum liquid chromatography with a gradient mobile phase was performed to obtain the purified extracts. The crude and purified extracts were observed to inhibit MCF-7 and HTB-183 cells using 3-(4,5- dimethyl thiazol 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The antioxidant properties of samples were determined using radical DPPH scavenging activity. The data was collected and analyzed to provide the inhibitory concentration 50 (IC50) and Pearson’s correlation was analyzed to describe the correlation between cytotoxic and antioxidant properties. Five purified extracts were obtained and tested against MCF-7 and HTB-183. The purified extract D has stronger anticancer and antioxidant activities than other samples with IC50 of 56.26 ± 3.11 µg/mL, 70.94 ± 2.92 µg/mL,  and 53.65 ± 1.55 µg/mL, respectively (p < 0.05). We confirmed that cytotoxic activity has a strong correlation with antioxidant properties. Furthermore, the active compounds of purified extract D needed to be investigated and tested against MCF-7 and HTB-183 to explore the possible anticancer mechanisms.

Graphical Abstract

Cytotoxic activity of the purified extracts from duku (Lansium domesticum Corr.) Leaf against MCF-7 and HTB-183 cell lines, and the correlation with antioxidant activity

Keywords

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